Two quick comments:

1. If you're reloading the scene that has the object flagged as 'don't destroy on load' in it, then a new copy of that object will be created every time the scene is loaded. (In other words, the fact that you flagged the object as 'don't destroy on load' doesn't prevent that object from being created again when the scene is reloaded.) If you only want one copy of that object, you'll need to set things up differently (for example, include the object in a 'startup' scene that's only loaded once).

2. Regarding the 'accessing transforms that have been destroyed' problem, be aware that any references the 'don't destroy on load' object is holding to game objects or components in the scene will become invalid when the scene reloads.

hi i haveing problem then i use appkication.loadlevel in my script it's automatecky loads next lvl from my main meniu lvl my script looks like this were i added application.loadlevel

private void DisplayButton() { if(GUI.Button(new Rect(Screen.width/ 2 - 50,STAT_STARTING_POINT + (10 * LINE_HEIGHT),STAT_LABLE_WIDTH,LINEHEIGHT),"Create")); { GameObject gs = GameObject.Find(" _gamesettings"); GameSettings gsScript = gs.GetComponent(); gsScript.SaveCharacterData(); Application.LoadLevel(1); } }