Should I change the Pixel To Unit ratio in a 2D Project?

Should I change the Pixel To Unit ratio in a 2D Project? I don’t think it affects anything that much.

However should i do it for a pixelated game? The default setting is set to 100, so for a let’s say, 64x64 sprite what should i set it to if this is even needed

it depends on your use case. If you want the sprite to be as big as one unit, you set the radio to 64, twice as big would be 32, but if you’re happy with the size as it is you just leave it as is.